Journal: bioRxiv
Article Title: Global genome analysis identifies glycolipids and lipoteichoic acid alanylation as contributors to Group B streptococcal diabetic wound infection
doi: 10.1101/2025.05.02.651901
Figure Lengend Snippet: Control (anti-IgG2a antibody, closed circles) and neutrophil depleted diabetic mice (anti-Ly6G antibody, open circles) were infected with 1 x 10 7 GBS WT, Δ iagB , and Δ dltA and bacterial burdens were assessed at 48h in the A) wound and B) kidney. Significantly higher bacterial burdens were recovered in the wound and kidney in depleted mice compared to control mice. C) % of mice in each group with CFU in the kidney. Control: GBS WT; n = 11, Δ iagB; n = 13, and Δ dltA; n = 12. Depleted: GBS WT; n = 12, Δ iagB; n = 13, and Δ dltA; n = 10. Significance determined by Kruskal-Wallis test with Dunn’s multiple comparisons; *p<.05, **p<.01, ****p<.0001.
Article Snippet: For neutrophil depletion experiments, mice were injected with 200 µg of either anti-Ly6G (BioXCell, clone 18A, catalog BE0075-1) or anti-IgG2a isotype control antibody (BioXCell, clone 2A3, catalog BE0089) diluted in buffered reagent diluent (BioXCell, catalog IP0070) the day before infection.
Techniques: Control, Infection